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Auralis Biotech S.r.l.
One-Step RT-PCR Master Mix
One-Step RT-PCR Master Mix
Simplified RT-PCR in a Single Tube – High-Efficiency, Lyophilized Format
The One-Step RT-PCR Master Mix is a lyophilized, ready-to-use reagent that enables reverse transcription and PCR amplification in a single reaction. Powered by M-MLV (H-) Reverse Transcriptase and hot-start Super HTaq DNA polymerase, this mix provides a robust, contamination-minimized solution for RNA detection workflows—ideal for gene expression studies, viral RNA detection, and molecular research.
Key Features
- One-Step Workflow: Reverse transcription and PCR combined into a single, streamlined protocol.
- High Sensitivity & Fidelity: Effective RNA detection from ≥100 ng input with full-length cDNA up to 2 kb.
- T-A Cloning Ready: Produces PCR products with A-overhangs for easy ligation into T-vectors.
- Lyophilized Convenience: No thawing or mixing required—just rehydrate and go.
- Flexible Primer Design: Compatible with sequence-specific primers or probes.
Applications
- Gene expression analysis by RT-PCR or qRT-PCR
- Detection of viral and microbial RNA
- Molecular cloning and sequencing
- cDNA generation for downstream applications
Product Specifications
| Catalog No. | Component | Specification | Quantity | Packaging |
|---|---|---|---|---|
| H015-1 | One-Step RT-PCR Master Mix | 48 reactions (20 μL each) | 48 Tubes (8-strip, 0.2 mL) | Pre-dispensed lyophilized tubes |
| PCR-grade water | 1.5 mL | 1 Tube (2 mL cryotube) | Supplied for reconstitution | |
| H015-2 | One-Step RT-PCR Master Mix | 500 reactions (20 μL each) | 1 Tube (bulk format) | Reconstitute with 5 mL PCR-grade water |
| PCR-grade water | 10 mL | 1 Tube | Supplied separately |
Storage & Transport
- Store sealed and dry at -25℃ to 8℃ for up to 24 months
- Room temperature stable (dry, ≤30% RH) for up to 9 months
- Transport below 30℃ for up to 2 months
Request a quote at info@auralisbiotech.it
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