Ready-to-Use 1st Strand cDNA Synthesis Master Mix

High-efficiency cDNA synthesis made easy – Ready-to-Use, Lyophilized Format

The Ready-to-Use 1st Strand cDNA Synthesis Master Mix is a premixed lyophilized reagent designed for rapid, reliable, and full-length first-strand cDNA synthesis from RNA templates. This high-performance mix features an optimized buffer system and robust M-MLV (H-) Reverse Transcriptase, making it ideal for downstream PCR, qPCR, cloning, and transcriptome analysis.

Key Features

• Complete Reverse Transcription System: Includes M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, MgCl₂, and reaction buffer.
• Wide Template Compatibility: Effective on RNA from animals, plants, and microorganisms.
• High Fidelity & Sensitivity: Capable of synthesizing full-length cDNA up to 2kb with excellent homogeneity.
• Flexible Primer Use: Supports Oligo(dT), random hexamers, or gene-specific primers (not included).
• Convenient Lyophilized Format: Ready for use without thawing or pre-mixing; ideal for single or batch reactions.
• Stable & Long Shelf Life: Stores up to 24 months at -25℃ to 8℃, or 9 months at room temperature in low moisture conditions.

Applications

• First-strand cDNA synthesis for RT-PCR and qPCR
• Gene expression studies
• Full-length cDNA library construction
• Primer extension and RNA mapping