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Auralis Biotech S.r.l.
Super easy SYBR®Green RT-qPCR Master mix
Super easy SYBR®Green RT-qPCR Master mix
One-Step RT-qPCR Solution with SYBR® Green – Just Add Template and Primers
The Super-Easy SYBR® Green RT-qPCR Master Mix is a ready-to-use lyophilized reagent designed for fast and sensitive one-step real-time RT-qPCR detection. It combines M-MLV (H-) Reverse Transcriptase and hot-start Super HP Taq DNA polymerase with SYBR® Green fluorescent dye in a single tube—ideal for high-throughput gene expression analysis and viral RNA detection.
Key Features
- True One-Step Reaction: Reverse transcription and qPCR amplification in one tube.
- SYBR® Green Fluorescent Detection: No probe design required—ideal for quantifying gene expression.
- High Sensitivity: Effective for low-copy RNA detection; stable performance with ≥100 ng RNA.
- Ready-to-Use Lyophilized Format: Add only primers, RNA, and water; ideal for automated or field workflows.
- Built-in dNTPs and Optimized Buffer: Formulated for robust, reproducible results.
Applications
- Gene expression quantification
- Viral and pathogen RNA detection
- High-throughput screening using SYBR® Green
- Basic and applied molecular biology research
Product Specifications
| Catalog No. | Component | Specification | Quantity | Packaging |
|---|---|---|---|---|
| H017-1 | Super-Easy SYBR® Green RT-qPCR Master Mix | 48 reactions (20 μL each) | 48 Tubes (8-strip, 0.2 mL) | Lyophilized mix in PCR tubes |
| PCR-grade water | 1.5 mL | 1 Tube (2 mL cryotube) | Supplied for reconstitution | |
| H017-2 | Super-Easy SYBR® Green RT-qPCR Master Mix | 500 reactions (20 μL each) | 1 Tube (bulk format) | Reconstitute with 5 mL PCR-grade water |
| PCR-grade water | 10 mL | 1 Tube | Supplied separately |
Storage & Transport
- Store sealed and dry at -25℃ to 8℃ for up to 24 months
- Stable at room temperature (dry, low humidity) for up to 9 months
- Transport below 30℃ for up to 2 months
- Protect from strong light during use and storage (due to SYBR® Green)
Request a quote at info@auralisbiotech.it
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