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Auralis Biotech S.r.l.

Super-Easy SYBR Green qPCR Master Mix

Super-Easy SYBR Green qPCR Master Mix

Streamlined Real-Time qPCR with Built-In SYBR® Green and dNTPs

The Super-Easy SYBR® Green qPCR Master Mix is a lyophilized, ready-to-use reagent for real-time quantitative PCR (qPCR) targeting DNA templates. With an optimized formulation that includes SYBR® Green fluorescent dye, hot-start Super HP Taq DNA polymerase, MgCl₂, and dNTPs, this mix simplifies qPCR workflows while ensuring high sensitivity and specificity.

Key Features

  • One-Tube SYBR® Green qPCR: Complete qPCR solution—just add DNA and primers.
  • Built-In SYBR® Green Dye: Enables real-time fluorescence detection without probes.
  • High Performance: Excellent amplification efficiency, low background, and strong signal intensity.
  • Ready-to-Use Lyophilized Format: Stable, pre-formulated tubes ideal for labs, classrooms, or field kits.
  • Ideal for Cloning: PCR products feature A-overhangs for direct T-vector ligation.

Applications

  • DNA quantification by real-time PCR
  • Gene expression analysis (from cDNA)
  • High-throughput screening and validation
  • Routine diagnostics and research workflows

Product Specifications

Catalog No. Component Specification Quantity Packaging
H016-1 Super-Easy SYBR® Green qPCR Master Mix 48 reactions (20 μL each) 48 Tubes (8-strip, 0.2 mL) Lyophilized mix in sealed PCR tubes
PCR-grade water 1.5 mL 1 Tube (2 mL cryotube) Supplied for reconstitution
H016-2 Super-Easy SYBR® Green qPCR Master Mix 500 reactions (20 μL each) 1 Tube (bulk format) Reconstitute with 5 mL PCR-grade water
PCR-grade water 10 mL 1 Tube Supplied separately

 

Storage & Transport

  • Store sealed and dry at -25℃ to 8℃ for up to 24 months
  • Stable at room temperature (dry, low humidity) for up to 9 months
  • Transport below 30℃ for up to 2 months
  • Protect from light during storage and use (SYBR® Green is light-sensitive)

Request a quote at info@auralisbiotech.it

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